IN VIVO
SCIENTIFIC & COMMERCIAL TRIALS
DONE WITH MYCO-AD®
SCIENTIFIC
TRIAL IN VIVO
MYCO-AD®
VS AFLATOXIN B1, 7.5 PPM WITH BROILERS
|
EXPERIMENTAL PROTOCOL
1. Animal: Broilers
2. Age: 4
days
3. Length: 20
days
4. Repetitions: 24
per treatment
5. Basic diet: Sorghum,
iso-caloric, iso-protein, iso-lysine soy, and with the same zurfacted AAC levels
6. Mycotoxin: Aflatoxin
B1, SIGMA LABS, USA
7. MYCO-AD® Standard
dosage of 2.5 kg / ton
|
TREATMENT
|
INITIAL WEIGHT
gr
|
FINAL WEIGHT
gr
|
CONSUMPTION
gr / day
|
GAIN
gr / day
|
FEED CONVERSION
|
|
CONTROL
|
78.87 a
|
639.47 a
|
45.90 a
|
28.03 a
|
1.637 a
|
|
CONTROL +
MYCO-AD®
|
78.35 a
|
634.95 a
|
47.82 a
|
27.83 a
|
1.717 a
|
|
CONTROL +
AF
|
76.71 a
|
446.51 b
|
35.42 b
|
18.49 b
|
1.915 b
|
|
CONTROL +
AF + MYCO-AD®
|
78.90 a
|
608.50 a
|
43.01 c
|
26.48 a
|
1.623 a
|
|
PROBABILITY
|
P < 0.052
|
P < 0.0001
|
P < 0.001
|
P < 0.001
|
P < 0.01
|
|
(*) Different
subscripts on a column indicate statistically significant differences
|
SCIENTIFIC
TRIAL IN VIVO
MYCO-AD®
VS OCHRATOXIN A, 2.0 PPM IN BROILERS
|
EXPERIMENTAL
PROTOCOL
1. Animal: Broilers
2. Age: 7 days
3. Length: 20
days
4. Repetitions: 16
per treatment
5. Basic diet: Sorghum,
iso-caloric, iso-protein, iso-lysine soy, and with the same zurfacted AAC levels
6. Mycotoxin: Ochratoxin
A, SIGMA LABS, USA
7. MYCO-AD® Standard
dosage of 2.5 kg / ton
|
TREATMENT
|
INITIAL WEIGHT
gr
|
FINAL WEIGHT
gr
|
CONSUMPTION
gr
/ day
|
GAIN
gr
/ day
|
FEED CONVERSION
|
|
CONTROL
|
82.45 a
|
734.5 a
|
53.87 a
|
31.05 a
|
1.734 a
|
|
CONTROL +
MYCO-AD®
|
85.36 a
|
738.8 a
|
54.85 a
|
31.12 a
|
1.762 a
|
|
CONTROL +
OC
|
82.63 a
|
705.5 a
|
52.91 a
|
29.67 b
|
1.783 a
|
|
CONTROL+OC
+ MYCO-AD®
|
85.19 a
|
770.4 a
|
53.89 a
|
32.63 a
|
1.651 b
|
|
PROBABILITY
|
P < 0.05
|
P < 0.05
|
P < 0.57
|
P < 0.05
|
P < 0.05
|
|
(*) Different
subscripts on a column indicate statistically significant differences
|
COMPARATIVE
SCIENTIFIC TRIAL IN VIVO
MYCO-AD®
VS PRODUCT "X" AND AFLATOXIN B1, 5.00 PPM WITH BROILERS
|
EXPERIMENTAL TRIAL
1. Animal: Broilers
2. Age: 11 days
3. Length: 16
days
4. Repetitions: 10
per treatment
5. Basic diet: Sorghum,
iso-caloric, iso-protein, iso-lysine soy, and with the same surfacted AAC levels
6. Mycotoxin: Aflatoxin
B1, SIGMA LABS, USA
7. MYCO-AD® Standard
dosage of 2.5 kg / ton
8. Product
"X" Dosage 2.5 kg / ton
|
TREATMENT
|
INITIAL WEIGHT
gr
|
FINAL WEIGHT
gr
|
CONSUMPTION
gr
/ day
|
GAIN
gr
/ day
|
FEED CONVERSION
|
|
CONTROL
|
258.7 a
|
845.3 a
|
68.82 a
|
41.44 a
|
1.680 a
|
|
CONTROL +
MYCO-AD®
|
256.0 a
|
863.4 a
|
67.75 a
|
40.48 a
|
1.673 a
|
|
CONTROL +
PRODUCT "x"
|
255.9 a
|
837.9 a
|
67.89 a
|
38.87 a
|
1.746 a
|
|
CONTROL +
AF
|
256.6 a
|
828.6 b
|
68.96 a
|
38.24 a
|
1.803 b
|
|
CONTROL +
AF + MYCO-AD®
|
255.3 a
|
893.0 a
|
71.37 b
|
42.48 a
|
1.680 a
|
|
CONTROL +
AF + PRODUCT "x"
|
252.4 a
|
822.7 b
|
69.26 a
|
38.01 b
|
1.822 b
|
|
PROBABILITY
|
P < 0.05
|
P < 0.05
|
P < 0.05
|
P < 0.05
|
P < 0.05
|
|
(*)
Different subscripts on a column indicate statistically significant differences
|
MYCO-AD®
VS PRODUCT "X",
CARCASS YIELD
|
TREATMENT
|
LEG YIELD
%
|
MUSCLE YIELD
%
|
BREAST YIELD
%
|
|
CONTROL
|
4.28 a
|
4.15 a
|
14.52 a
|
|
CONTROL +
MYCO-AD®
|
4.18 a
|
4.18 a
|
14.52 a
|
|
CONTROL +
PRODUCT "x"
|
4.19 a
|
4.47 a
|
14.94 a
|
|
CONTROL +
AF
|
4.12 a
|
4.05 a
|
14.44 a
|
|
CONTROL +
AF + MYCO-AD®
|
5.75 b
|
5.43 b
|
18.46 b
|
|
CONTROL +
AF + PRODUCT "x"
|
3.86 a
|
3.72 a
|
12.98 a
|
|
PROBABILITY
|
P < 0.05
|
P < 0.05
|
P < 0.05
|
|
(*) Different
subscripts on a column indicate statistically significant differences
|
|
COMMERCIAL TRIAL
BROILER FEED CONTAINING 2 ppm
T-2
|
Place: Mayor
Mexican Poultry Integrator
Type of animal: Broilers
Disease: Mycotoxicosis
by Trichothecenes at 2 ppm (T-2, DAS ... etc)
Trial start: 4th
week: when 100% of the birds had oral lesions
Dosage MYCO-AD®
was added at 2.5 kg / ton
COMPARATIVE COMMERCIAL TRIAL
WITH COMMERCIAL FEED
MYCO-AD®
VS A COMMERCIAL ALUMINOSILICATE IN BROILERS
|
In a field trial performed
in Guerrero (México), where MYCO-AD® at 2.5 kg
/ ton of feed was compared to a commercial aluminosilicate at 5
kg / ton of feed, feeding commercial broiler flocks from 1 to 56 days of age,
under a clinical suspicions of exposure to mycotoxins, the following was found:
PRODUCTIVE RESULTS OF
BROILER FLOCKS TREATED WITH MYCO-AD® AND ANOTHER COMMERCIAL ALUMINOSILICATE
|
ZOOTECHNICAL
PARAMETERS
8th week
|
MYCO-AD®
2.5 kg / ton
|
ALUMINOSILICATE
5.0 kg / ton
|
|
MORTALITY
|
10.78
%
|
11.55 %
|
|
FEED CONVERSION
|
2.39
|
2.44
|
|
FINAL
WEIGHT
|
2.570
kg
|
2.240 kg
|
|
DAILY
GAIN
|
45.89
g
|
40.0 g
|
|
PRODUCTIVITY
INDEX
|
175
|
156
|
COMPARATIVE SCIENTIFIC TRIAL
WITH COMMERCIAL FEED
WITH AFLATOXIN 100 / 140 PPB AND T2 100 / 150 PPB IN LAYERS
|
Three groups
of 100 white Babcock layers, 30 weeks of age, housed under commercial conditions,
were given MYCO-AD® at a 2.5 kg / ton of feed, a synthetic
aluminum silicate at 1.0 Kg / ton and a group was used as non medicated
control. The commercial feed contained 100 to 140 ppb of aflatoxin and
100 to 150 ppb of T2. After 19 days of trial, statistical analysis
of the results were performed:
MYCO-AD
VS SYNTHETIC ALUMINOSILICATE IN LAYERS
|
GROUP
|
EGG WEIGHT
gr
|
PRODUCTION
%
|
EGG MASS
kg
|
FEED CONVERSION
|
|
CONTROL
|
56.9 a
|
88.4 a
|
95.5 a
|
2.161 a
|
|
MYCO-AD®
|
58.4 b
|
93.2 b
|
103.4
b
|
2.000
b
|
|
SYNTHETIC
|
55.9 a
|
90.5 a
|
96.0 a
|
2.126 a
|
|
PROBABILITY
|
P < 0.05
|
P < 0.05
|
P < 0.05
|
P < 0.05
|
|
(*) Different
subscripts on a column indicate statistically significant differences
|
SCIENTIFIC TRIAL WITH
COMMERCIAL FEED
TO
PROVE THAT MYCO-AD®
DOES NOT ABSORB NUTRIENTS
|
Trial performed
at CEIEPA, FMVZ, UNAM, using a fully random design, with 432 Isa Babcock B-300
Layers, 30 weeks of age, housed in cages of 3 each, 2 treatments, were used with
6 repetitions of 12 layers each. The following treatments were provided: control
base diet, base diet + MYCO-AD® at 2.5 kg / Mt, and the following
results were obtained:
MYCO-AD®
IN LAYERS WITH NO EXPOSURE
TO MYCOTOXINS
|
TREATMENT
|
LAYING
%
|
EGG WEIGHT
gr
|
CONSUMPTION
gr
|
FEED CONV
|
BIRD /
DAY
MASS
gr
|
HAUGH
UNIT
|
SHELL
WITH
mm
|
|
CONTROL
|
82.5 a
|
61.5 a
|
104.2 a
|
2.075 a
|
50.8 a
|
90.67 a
|
0.322 a
|
|
MYCO-AD®
|
81.8 a
|
60.9 a
|
103.1
a
|
2.073
a
|
49.8 a
|
89.13
a
|
0.325
a
|
|
PROBABILITY
|
P < 0.05
|
P < 0.05
|
P < 0.05
|
P < 0.05
|
P < 0.05
|
P < 0.05
|
P < 0.05
|
|
(*) Different
subscripts on a column indicate statistically significant differences
|
SCIENTIFIC TRIAL
1 PPM OF T-2 IN BROILERS VS.
MYCO-AD®
|
|
TREATMENT
|
INITIAL
WEIGHT
|
FINAL
WEIGHT
|
DAILY
CONSUMPTION
|
AVERAGE
DAILY
WEIGHT
|
FEED CONSUMPTION
|
|
Control
|
42 a
|
1791 a
|
95 a
|
43,73 a
|
2,12 a
|
|
Control +
1 ppm T2
|
41 a
|
138 lb
|
75 b
|
33,50 b
|
2,17 a
|
|
Control +1
ppm T-2 + 2.5 kg Myco-Ad
|
42 a
|
1840 a
|
95 a
|
44,95 a
|
2,06 a
|
|
Probability
|
P <0.05
|
P<0.05
|
P<0.05
|
P<0.05
|
P<0.05
|
|
(*) Different subscripts
in a column indicates statistically significant difference.
Institute of Veterinary
Medicine, Debrecen, Hungary, 1999
|
ORAL LESIONS
SEVERITY OF ORAL
LESIONS
|
Group
II
per day
|
0
|
21
|
28
|
35
|
40
|
|
Negative
|
25
|
3
|
3
|
11
|
18
|
|
+
|
0
|
6
|
10
|
6
|
4
|
|
++
|
0
|
8
|
4
|
2
|
1
|
|
+++
|
0
|
8
|
7
|
4
|
0
|
|
Group
II
per day
|
0
|
21
|
28
|
35
|
40
|
|
Negative
|
25
|
18
|
20
|
22
|
23
|
|
+
|
0
|
5
|
3
|
1
|
0
|
|
++
|
0
|
2
|
1
|
0
|
0
|
|
+++
|
0
|
0
|
0
|
0
|
0
|
SKELETON DISORDER / % /
RESULTS
AT THE END OF THE TRIAL:
The presence of
T-2 (Group II) caused:
• Less body weight
by 23 %
• Less feed consumption
by 21 %
• Necrosis of the
mucous and skin of the oral cavity
• Atrophy of the
Limphotic organs by 25 % to 50 %
• In groups I y II
there were problems in the feet in 18 % of the birds.
SCIENTIFIC TRIAL
MYCO-AD®
VS 50 PPB OF AFLATOXIN B1 IMMUNOSUPRESION
LOW TITERS IN BROILERS
|
|
Control
|
28 days
|
35 days
|
42 days
|
|
50 ppb Afla
|
20.2 gmt
|
17.6 gmt.
|
15.2 gmt.
|
|
Myco-Ad®
50 ppb Afla
|
35.2 gmt
|
41.6 gmt.
|
46.4 gmt.
|
|
Control
|
Yield or
gain
|
Consumption
|
FCR
|
|
Control 50
ppb Afla
|
1726.7 gr
|
3930
|
227
|
|
Control +
Myco-Ad® + 50 ppb Afla
|
1861.7 gr
|
3976
|
213
|
|
Pakistan, 1999
|
SCIENTIFIC
TRIAL
MYCO-AD®
IN SWINE PRODUCTION
|
|
|
T1
|
T2
|
T3
|
|
|
Control
with MB
|
Myco-Ad®
|
Commercial
MB
|
|
Nº of animals/
treatment
|
48
|
48
|
48
|
|
Nº of replicas/
treatment
|
4
|
4
|
4
|
|
Male/Female
ratio
|
24/24
|
24/24
|
24/24
|
|
Days of feeding
|
40
|
40
|
40
|
|
Average (Avg)
initial weight (wt/kg)
|
26.47
|
27
|
26.62
|
|
Avg. Final
wt
|
49.81
|
50.51
|
49.91
|
|
Average change
in body wt (kg)
|
23.34
|
23.51
|
23.29
|
|
Avg, daily
gain (grams)
|
583.5
|
587.75
|
582.25
|
|
Avg. Feed
consumption(kg)
|
1.48 a
|
1.44 c
|
1.45 b
|
|
Avg. Feed
Conversion
|
2.54
|
245
|
2.49
|
|
COST BENEFIT
|
|
Cost of HSM
(in Pesos)
|
8.47
|
8.66
|
8.68
|
|
Feed Efficiency
|
2.54
|
2.45
|
2.49
|
|
Cost of pork
kg/gain
|
21.51
|
21.21
|
21.61
|
|
Savings/
kg gain vs. control
|
0
|
0.3
|
-0.1
|
|
The levels
of mycotoxins were 47 ppb of Aflatoxin, 8 ppb of Ochratoxin, 5 ppb of T-2 and
125 ppb of Zearalenone.
(1)Phillippines,
1999
Means with different
superscripts are significantly different (<P0.05)
|
REDUCTION
OF AFLATOXIN M1
IN MILK BY USING
MYCO-AD®
Place: Italy
Customer: World
known Feed Manufacturer
The European regulations
admit only a maximum of 50 ppt of Aflatoxin M1 in Milk. This means
that the level of Aflatoxin in feed should be a maximum of 5 ppb.
Due to Silage problems this
year the contamination of Aflatoxin has been much higher than the normal. Also
the government is now emphasizing checking the levels of Aflatoxin M1 in
milk.
The total amount of cows
in this trial were 800 before the administration of MYCO-AD®.
The levels of M1 were between 75 to 60 ppt which are above the levels
allowed by the European Common Market.
The treatment with MYCO-AD®
at 50 grams/head per day started on April 17th, 1999, and the results
of reduction were seen immediately. Throughout the rest of the trial the levels
of M1 were below the maximum levels allowed, so the milk was in accordance
to regulations.
Reduction
of Aflatoxin M1 in Milk by Using Myco-Ad
During the trial the Nutritionist
also observed that there was a big relationship between the level of M1
and the somatic cells count. The higher the M1 the higher the somatic
cells count. Because of this, even at low levels of Aflatoxin 5 ppb it was affecting
the animal immune system.
